Study of Epilobium angustifolium L. amino acids content by HPLC method

The use of plant raw materials is one the areas modern pharmaceutical science in production herbal drugs. genus Epilobium counts more than 200 species, many species which are used traditional medicine. Among angustifolium well-known medicinal plants have been worldwide habitual There insufficient information literature on biologically active substances L. presence three major polyphenol groups: phenolic acids, flavonoids, and ellagitannins were identified E. extracts. Traditionally, infusion leaves this could be useful for headaches, cold gastrointestinal disorder. as an insufficiently studied a promising object study, including amino acids composition. To assess relationship between primary metabolites their possible therapeutic properties, we analyzed acid profile study compounds generated by result defense mechanisms permits understanding molecular mechanism involved properties.
 aim. Thus, aim was to conduct HPLC analysis establish prospects medical practice. results current will further breeding programs aimed obtain industrial form suitable food applications.
 Materials methods. determination composition conducted using Agilent 1200 (Agilent Technologies, USA).
 Results. method sixteen free seventeen bound herb. studies shown that herb mainly composed such L-phenylalanine (1.65 µg/mg), L-glutamic (1.51 L-arginine (1.24 L-alanine (0.98 µg/mg) L-aspartic (0.57 presents greatest amount. dominant material acid, L-leucine, L-alanine, content 32.37 µg/mg, 10.59 8.70 6.22 µg/mg respectively.
 Conclusions. Using determined concentrations L-arginine, predominate among shows source so new remedies future